Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods. 2011 Feb;53(2):175-84. doi: 10.1016/j.ymeth.2010.07.006. Epub 2010 Jul 16.

Comprehensive analysis of DNA-methylation in mammalian tissues using MeDIP-chip.

Author information

1
Institut für Genetik, FB Biowissenschaften, Universität des Saarlandes, Saarbrücken, Germany.

Abstract

Genome-wide mapping of epigenetic changes is essential for understanding the mechanisms involved in gene regulation during cell differentiation and embryonic development. DNA-methylation is one of these key epigenetic marks that is directly linked to gene expression is. Methylated DNA immunoprecipitation (MeDIP) is a recently devised method used to determine the distribution of DNA-methylation within functional regions (e.g., promoters) or in the entire genome robustly and cost-efficiently. This approach is based on the enrichment of methylated DNA with an antibody that specifically binds to 5-methyl-cytosine and can be combined with PCR, microarrays or high-throughput sequencing. This article outlines the experimental procedure of MeDIP-chip and provides a comprehensive summary of quality control strategies and primary data analysis.

PMID:
20638478
DOI:
10.1016/j.ymeth.2010.07.006
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center