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J Magn Reson. 2010 Sep;206(1):105-11. doi: 10.1016/j.jmr.2010.06.011. Epub 2010 Jul 1.

1H-13C separated local field spectroscopy of uniformly 13C labeled peptides and proteins.

Author information

1
Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307, USA.

Abstract

By incorporating homonuclear decoupling on both the (1)H and (13)C channels it is feasible to obtain high-resolution two-dimensional separated local field spectra of peptides and proteins that are 100% labeled with (13)C. Dual-PISEMO (Polarization Inversion Spin Exchange Modulated Observation) can be performed as a conventional two-dimensional experiment, or with windowed detection as a one-dimensional experiment that offers flexibility as a building block for shiftless and other multidimensional triple-resonance experiments with the inclusion of (15)N irradiation. The triple-resonance MAGC probe used to perform these experiments at 500 MHz is described.

PMID:
20637662
PMCID:
PMC2926282
DOI:
10.1016/j.jmr.2010.06.011
[Indexed for MEDLINE]
Free PMC Article

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