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Annu Rev Anal Chem (Palo Alto Calif). 2008;1:423-49. doi: 10.1146/annurev.anchem.1.031207.113042.

Cell culture models in microfluidic systems.

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Bellbrook Labs, LLC, Madison, Wisconsin 53711, USA.


Microfluidic technology holds great promise for the creation of advanced cell culture models. In this review, we discuss the characterization of cell culture in microfluidic systems, describe important biochemical and physical features of the cell microenvironment, and review studies of microfluidic cell manipulation in the context of these features. Finally, we consider the integration of analytical elements, ways to achieve high throughput, and the design constraints imposed by cell biology applications.

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