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Annu Rev Anal Chem (Palo Alto Calif). 2008;1:165-90. doi: 10.1146/annurev.anchem.1.031207.113104.

Chemical cytometry: fluorescence-based single-cell analysis.

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1
Department of Chemistry, University of Washington, Seattle, 98195, USA.

Abstract

Cytometry deals with the analysis of the composition of single cells. Flow and image cytometry employ antibody-based stains to characterize a handful of components in single cells. Chemical cytometry, in contrast, employs a suite of powerful analytical tools to characterize a large number of components. Tools have been developed to characterize nucleic acids, proteins, and metabolites in single cells. Whereas nucleic acid analysis employs powerful polymerase chain reaction-based amplification techniques, protein and metabolite analysis tends to employ capillary electrophoresis separation and ultrasensitive laser-induced fluorescence detection. It is now possible to detect yoctomole amounts of many analytes in single cells.

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