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Mediators Inflamm. 2010;2010:813610. doi: 10.1155/2010/813610. Epub 2010 Jun 20.

Induction of interleukin-8 from nasal epithelial cells during bacterial infection: the role of IL-8 for neutrophil recruitment in chronic rhinosinusitis.

Author information

1
Department of ORL-HNS and Medical Research Institute, Pusan National University School of Medicine, Beomeo-ri, Mulgeum-eup, Yangsan-si, Gyeongsangnam-do 626-770, Republic of Korea.

Abstract

OBJECTIVES:

The aim of this study was to elucidate the role of IL-8 for neutrophil recruitment in nonallergic CRS patients.

METHODS:

After coculture of Streptococcus pneumoniae (SP) with the mucosal epithelial cells (MECs) from non-CRS patients, at three different SP/MEC (1/1, 10/1, 100/1) ratios, the expression of IL-8 mRNA and the concentration of IL-8 were measured by RT-PCR and ELISA. The expression of CD11b/CD18 on neutrophils and E-selectin/ICAM-1 on endothelial cells and the adherence between neutrophils and human umbilical vascular endothelial cells (HUVECs) were determined by flow cytometric analysis, ELISA, and RIA, respectively.

RESULTS:

IL-8 concentration and IL-8 mRNA expression continued to increase from 3 hours after incubation in SP number-dependent manner. The expression of CD11b/CD18 on neutrophils and E-selectin/ICAM-1 on HUVECs, and the adherence between neutrophils and HUVECs were significantly increased in 10 SP/MEC-CM, and the increments were significantly blocked by anti-IL-8 antibody.

CONCLUSION:

MEC and IL-8 are major factors for neutrophil recruitment in nonallergic CRS.

PMID:
20634942
PMCID:
PMC2904024
DOI:
10.1155/2010/813610
[Indexed for MEDLINE]
Free PMC Article

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