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Br J Cancer. 2010 Aug 10;103(4):517-23. doi: 10.1038/sj.bjc.6605786. Epub 2010 Jul 13.

Secretion of intelectin-1 from malignant pleural mesothelioma into pleural effusion.

Author information

1
Division of Cancer Therapy, Kanagawa Cancer Center Research Institute, 1-1-2 Nakao, Asahi-ku, Yokohama-shi, Kanagawa 241-0815, Japan. stsuji@gancen.asahi.yokohama.jp

Abstract

BACKGROUND:

Malignant pleural mesothelioma (MPM) is a rare but fatal tumour. Although most MPM patients show pleural effusion at even the early stage, it is hard to diagnose as MPM at the early stage because a sensitive and reliable diagnostic marker for MPM has not been found in plasma or pleural effusion.

METHODS:

In this study, we investigated whether intelectin-1 was specifically contained in MPM cells and the pleural effusion of MPM patient by immunohistochemistry, western blotting, and enzyme-linked immunosorbent assay.

RESULTS:

Malignant pleural mesothelioma cell lines, but not lung adenocarcinoma cell lines, secreted intelectin-1. In immunohistochemistry, epithelioid-type MPMs, but neither pleura-invading lung adenocarcinomas nor reactive mesothelial cells near the lung adenocarcinomas, were stained with anti-intelectin antibodies. Pleural effusion of MPM patients contained a higher concentration of intelectin-1 than that of lung cancer patients.

CONCLUSION:

These results suggest that detection of intelectin-1 may be useful for a differential diagnosis of epithelioid-type MPM in immunohistochemistry and that a high concentration of intelectin-1 in pleural effusion can be used as a new marker for clinical diagnosis of MPM.

PMID:
20628387
PMCID:
PMC2939784
DOI:
10.1038/sj.bjc.6605786
[Indexed for MEDLINE]
Free PMC Article

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