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J Heart Lung Transplant. 2010 Sep;29(9):1071-5. doi: 10.1016/j.healun.2010.04.023. Epub 2010 Jun 8.

Epoprostenol-associated pneumonitis: diagnostic use of a T-cell proliferation assay.

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1
Division of Pulmonary and Critical Care Medicine, Stanford University, Stanford, California, USA. kkudelko@stanford.edu

Abstract

We describe a case of severe drug-induced interstitial pneumonitis in a woman with idiopathic pulmonary arterial hypertension receiving epoprostenol confirmed by a drug T-cell proliferation assay. Proliferation assays were completed in our patient and in a healthy control. Isolated T cells were incubated with CD3-depleted peripheral blood mononuclear cells and then stimulated to proliferate with (3)H-thymidine in the presence of epoprostenol, other prostanoid analogs, and controls. A significant (p < 0.001) T-cell proliferation response occurred in our patient in the presence of epoprostenol alone. There was a trend towards an increased T-cell response to treprostinil but this was statistically insignificant. There was no significant T-cell response to the diluent alone, normal saline, iloprost, or alprostadil. There was no significant proliferation to any drug in the healthy control. Hence, a drug T-cell proliferation assay confirmed that epoprostenol can rarely incite a profound inflammatory response in the pulmonary interstitium.

PMID:
20627625
PMCID:
PMC2926193
DOI:
10.1016/j.healun.2010.04.023
[Indexed for MEDLINE]
Free PMC Article
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