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Inflamm Res. 2011 Jan;60(1):19-27. doi: 10.1007/s00011-010-0230-4. Epub 2010 Jul 13.

IgE-dependent sensitization increases responsiveness to LPS but does not modify development of endotoxin tolerance in mast cells.

Author information

1
Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados del IPN, Sede Sur, Calzada de los Tenorios No 235, Col Granjas Coapa, Tlalpan, Mexico, DF, México.

Abstract

OBJECTIVE:

Effects of immunoglobulin E (IgE)-dependent sensitization on the response to bacterial lipopolysaccharide (LPS) were analyzed in mast cells.

METHODS:

Murine bone marrow-derived mast cells (BMMCs) were sensitized or not with IgE before stimulation with LPS. TLR4 and co-receptors expression was analyzed by flow cytometry and RT-PCR, TNF-α production by ELISA, IKK and IκB activation by western blot or immunoprecipitation. NFκB nuclear translocation was determined by EMSA.

RESULTS:

IgE-sensitized BMMCs secreted larger amounts of TNF-α than non-sensitized cells shortly after LPS challenge. No change in TLR4, CD14 or MD-2 expression was detected after the IgE-dependent sensitization process, whereas TLR4-dependent phosphorylation of IKK and IκB was augmented. IgE-dependent sensitization increased basal NFκB activity. Endotoxin tolerance was not affected by the IgE-dependent sensitization process.

CONCLUSIONS:

IgE-induced sensitization primes mast cells for higher response to LPS through pre-activation of NFκB transcription factor. IgE-dependent sensitization does not modify events leading to endotoxin tolerance.

PMID:
20625918
DOI:
10.1007/s00011-010-0230-4
[Indexed for MEDLINE]

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