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Nat Chem Biol. 2010 Aug;6(8):615-20. doi: 10.1038/nchembio.406. Epub 2010 Jul 11.

n-->pi* interactions in proteins.

Author information

1
School of Chemistry, University of Bristol, Bristol, United Kingdom.

Abstract

Hydrogen bonds between backbone amides are common in folded proteins. Here, we show that an intimate interaction between backbone amides also arises from the delocalization of a lone pair of electrons (n) from an oxygen atom to the antibonding orbital (pi*) of the subsequent carbonyl group. Natural bond orbital analysis predicted significant n-->pi* interactions in certain regions of the Ramachandran plot. These predictions were validated by a statistical analysis of a large, non-redundant subset of protein structures determined to high resolution. The correlation between these two independent studies is striking. Moreover, the n-->pi* interactions are abundant and especially prevalent in common secondary structures such as alpha-, 3(10)- and polyproline II helices and twisted beta-sheets. In addition to their evident effects on protein structure and stability, n-->pi* interactions could have important roles in protein folding and function, and merit inclusion in computational force fields.

PMID:
20622857
PMCID:
PMC2921280
DOI:
10.1038/nchembio.406
[Indexed for MEDLINE]
Free PMC Article

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