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Vaccine. 2010 Aug 23;28(37):6052-7. doi: 10.1016/j.vaccine.2010.06.091. Epub 2010 Jul 7.

Exploiting knowledge of immune selection in HIV-1 to detect HIV-specific CD8 T-cell responses.

Author information

1
Centre for Clinical Immunology and Biomedical Statistics, Institute of Immunology and Infectious Diseases, Murdoch University, Perth, Western Australia, Australia.

Abstract

Since HLA-restricted cytotoxic T-cell responses select specific polymorphisms in HIV-1 sequences and HLA diversity is relatively static in human populations, we investigated the use of peptide epitopes based on sites of HLA-associated adaptation in HIV-1 sequences to stimulate and detect T-cell responses ex vivo. These "HLA-optimised" peptides captured more HIV-1 Nef-specific responses compared with overlapping peptides of a single consensus sequence, in interferon-gamma enzyme linked immunospot assays. Sites of immune selection can reveal more immunogenic epitopes in HLA-diverse populations and offer insights into the nature of HLA-epitope targeting, which could be applied in vaccine design.

PMID:
20619380
PMCID:
PMC2949439
DOI:
10.1016/j.vaccine.2010.06.091
[Indexed for MEDLINE]
Free PMC Article

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