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J Biochem Biophys Methods. 1991 Feb-Mar;22(2):159-65.

The human multicatalytic proteinase: affinity purification using a monoclonal antibody.

Author information

1
August Krogh Institute, Copenhagen, Denmark.

Abstract

A monoclonal antibody, coupled to Sepharose CL-4B, was used for the rapid purification of the human multicatalytic proteinase in a single chromatographic step under mild conditions. The enzyme was homogeneous as judged by nondenaturing polyacrylamide gel electrophoresis. Electrophoresis under dissociating and reducing conditions revealed at least ten components with molecular masses in the range 22-34 kDa. Affinity-purified enzyme was identical to conventionally purified enzyme with respect to enzymatic properties, molecular mass and subunit composition.

PMID:
2061562
DOI:
10.1016/0165-022x(91)90028-u
[Indexed for MEDLINE]

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