Format

Send to

Choose Destination
J Biomed Opt. 2010 May-Jun;15(3):030505. doi: 10.1117/1.3420209.

Multiphoton microscopy with near infrared contrast agents.

Abstract

While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

PMID:
20614991
PMCID:
PMC2881927
DOI:
10.1117/1.3420209
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Society of Photo-Optical Instrumentation Engineers Icon for PubMed Central
Loading ...
Support Center