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World J Gastroenterol. 2010 Jul 14;16(26):3258-66.

CD74 is a survival receptor on colon epithelial cells.

Author information

1
Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel.

Abstract

AIM:

To investigate the expression and function of CD74 in normal murine colon epithelial cells (CEC) and colon carcinoma cells.

METHODS:

Expression of CD74 mRNA and protein were measured by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and fluorescence-activated cell sorter (FACS). The effect of migration inhibitory factor (MIF) on the survival of normal CEC from C57BL/6, NOD/SCID, and CD74 deficient mice both in vitro and in vivo, and on the CT26 carcinoma cell line was analyzed by (quantitative) qRT-PCR, RT-PCR, Western blotting and FACS.

RESULTS:

CD74 was found to be expressed on normal CEC. Stimulation of CD74 by MIF induced a signaling cascade leading to up-regulation of Bcl-2 expression, resulting in a significant increased survival of CEC. CD74 was also expressed on the CT26 colon carcinoma cell line and its stimulation by MIF resulted in enhanced cell survival, up-regulation of Akt phosphorylation and Bcl-2 expression.

CONCLUSION:

CD74 is expressed on CEC and colon carcinoma cells and serves as a survival receptor in these cells. These results may have implications on colorectal cancer research.

PMID:
20614481
PMCID:
PMC2900717
[Indexed for MEDLINE]
Free PMC Article

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