Format

Send to

Choose Destination
Dev Genes Evol. 1997 May;207(1):19-28. doi: 10.1007/s004270050088.

Inhibitory control of neural differentiation in mammalian cells.

Author information

1
The Rockefeller University, 1230 York Avenue, New York, NY 10021-6339, USA, , , , , , US.

Abstract

In Xenopus embryos, a truncated type II activin receptor (Delta1XAR1), capable of blocking signals by several transforming growth factor (TGF)-beta family members, can induce neural tissue suggesting neural fate is under inhibitory control. Activin and bone morphogenetic protein 4 (BMP4) can act as neural inhibitors but only BMP4 can induce epidermis in Xenopus ectodermal cells. We have used the pluripotent mouse embryonal carcinoma cell line P19 to examine whether the mechanisms of ectodermal cell fate decisions are conserved among vertebrates. We show that a P19 cell line expressing Delta1XAR1 will differentiate into neurons. In addition, BMP4 inhibits retinoic acid (RA)-induced neural differentiation of P19 cells and induces keratin expression. These results suggest that in mammals as in amphibians neural fate is under inhibitory control and BMP4 can alter ectodermal differentiation.

PMID:
20607477
DOI:
10.1007/s004270050088

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center