Format

Send to

Choose Destination
Cell. 2010 Jul 9;142(1):158-69. doi: 10.1016/j.cell.2010.05.037.

A comprehensive comparison of transmembrane domains reveals organelle-specific properties.

Author information

1
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK.

Abstract

The various membranes of eukaryotic cells differ in composition, but it is at present unclear if this results in differences in physical properties. The sequences of transmembrane domains (TMDs) of integral membrane proteins should reflect the physical properties of the bilayers in which they reside. We used large datasets from both fungi and vertebrates to perform a comprehensive comparison of the TMDs of proteins from different organelles. We find that TMDs are not generic but have organelle-specific properties with a dichotomy in TMD length between the early and late parts of the secretory pathway. In addition, TMDs from post-ER organelles show striking asymmetries in amino acid compositions across the bilayer that is linked to residue size and varies between organelles. The pervasive presence of organelle-specific features among the TMDs of a particular organelle has implications for TMD prediction, regulation of protein activity by location, and sorting of proteins and lipids in the secretory pathway.

PMID:
20603021
PMCID:
PMC2928124
DOI:
10.1016/j.cell.2010.05.037
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center