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J Microbiol Methods. 2010 Sep;82(3):234-7. doi: 10.1016/j.mimet.2010.06.005. Epub 2010 Jun 21.

The antibacterial potency of the medicinal maggot, Lucilia sericata (Meigen): variation in laboratory evaluation.

Author information

1
School of Natural and Applied Sciences, University of Lincoln, Brayford Pool, Lincoln, LN6 7TS, UK. K.Barnes@shu.ac.uk

Abstract

Research to quantify the potency of larval excretion/secretion from Lucilia sericata using liquid culture assays has produced contradictory results. In this study, viable counting was used to investigate the effectiveness of excretion/secretion against three marker bacterial species (Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli) and the effects of varying growing conditions in assays. Results demonstrate that factors such as number of larvae, species of bacteria and addition of nutrient influence its antibacterial potency. Therefore a standardised method should be employed for liquid culture assays when investigating the antibacterial activity of larval excretion/secretion from L. sericata.

PMID:
20600366
DOI:
10.1016/j.mimet.2010.06.005
[Indexed for MEDLINE]

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