Send to

Choose Destination
J Neurosci Methods. 2010 Aug 30;191(2):151-7. doi: 10.1016/j.jneumeth.2010.06.012. Epub 2010 Jun 17.

Characterization of the hemodynamic response in the rat lumbar spinal cord using intrinsic optical imaging and laser speckle.

Author information

Département de génie électrique, Ecole Polytechnique de Montréal, Montreal, QC, Canada.


Quantifying spinal cord functions is crucial for understanding neurophysiological mechanisms governing the intact and the injured spinal cord. Intrinsic optical imaging (IOI) and laser speckle provides measures of deoxyhemoglobin (HbR) and oxyhemoglobin (HbO(2)) concentrations, blood volume (BV) and blood flow (BF) at high spatial and temporal resolution. In this study we used IOI and laser speckle to characterize the hemodynamic response to neuronal activation in the lumbar spinal cord of anaesthetized rats (N=9). We report consistent temporal variations of HbR, HbO(2), BV and BF located ipsilaterally at L3-L5. Responses were significantly higher when stimulation intensity was increased. Vascular changes extended several millimetres from the epicenter, supporting the venous drainage observed in functional magnetic resonance imaging studies.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center