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Org Lett. 2010 Aug 6;12(15):3300-3. doi: 10.1021/ol100923d.

Hoechst-IR: an imaging agent that detects necrotic tissue in vivo by binding extracellular DNA.

Author information

1
The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.

Abstract

Cell necrosis is central to the progression of numerous diseases, and imaging agents that can detect necrotic tissue have great clinical potential. We demonstrate here that a small molecule, termed Hoechst-IR, composed of the DNA binding dye Hoechst and the near-infrared dye IR-786, can image necrotic tissue in vivo via fluorescence imaging. Hoechst-IR detects necrosis by binding extracellular DNA released from necrotic cells and was able to image necrosis generated from a myocardial infarction and lipopolysaccharide/d-galactosamine (LPS-GalN) induced sepsis.

PMID:
20597468
PMCID:
PMC2929653
DOI:
10.1021/ol100923d
[Indexed for MEDLINE]
Free PMC Article

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