Format

Send to

Choose Destination
Cell Adh Migr. 2010 Oct-Dec;4(4):491-501.

Use of photoactivation and photobleaching to monitor the dynamic regulation of E-cadherin at the plasma membrane.

Author information

1
Edinburgh Cancer Research Centre, University of Edinburgh, Edinburgh, UK. m.canel@ed.ac.uk

Abstract

The dynamic control of E-cadherin is critical for establishing and maintaining cell-cell junctions in epithelial cells. The concentration of E-cadherin molecules at adherens junctions (AJs) is regulated by lateral movement of E-cadherin within the plasma membrane and endocytosis. Here we set out to study the interplay between these processes and their contribution to E-cadherin dynamics. Using photoactivation (PA) and fluorescence recovery after photobleaching (FRAP) we were able to monitor the fate of E-cadherin molecules within the plasma membrane. Our results suggest that the motility of E-cadherin within, and away from, the cell surface are not exclusive or independent mechanisms and there is a fine balance between the two which when perturbed can have dramatic effects on the regulation of AJs.

PMID:
20595808
PMCID:
PMC3011267
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center