Format

Send to

Choose Destination
Genes Dev. 2010 Jul 1;24(13):1345-50. doi: 10.1101/gad.576310.

Novel role for a bacterial nucleoid protein in translation of mRNAs with suboptimal ribosome-binding sites.

Author information

1
Department of Molecular Biology, Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden. hyun-sook.park@molbiol.umu.se

Abstract

In Escherichia coli, the major nucleoid protein H-NS limits transcription by acting as a repressor or transcriptional silencer, presumably by its ability to close the looped chromosome domains in the nucleoid through DNA-protein-DNA bridging. Here, we demonstrate the direct involvement of H-NS as a positive factor stimulating translation of the malT mRNA. In vitro studies showed that H-NS facilitates a repositioning of the 30S preinitiation complex on the malT mRNA. H-NS stimulation of translation depended on the AU-rich -35 to -40 region of the mRNA. Several additional examples were found demonstrating a novel function for H-NS in translation of genes with suboptimal ribosome-binding sequences.

PMID:
20595230
PMCID:
PMC2895194
DOI:
10.1101/gad.576310
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center