Abstract
The role of retinoblastoma protein-interacting zinc finger 1 (RIZ1) on the cell growth of mouse and human monocytic leukemia cells was examined. RIZ1 expression was induced in response to tumor necrosis factor (TNF)-α. The expression was dependent on the nuclear factor-κB and AKT signaling. Further, RIZ1 expression led to the augmentation of p53 expression and the silencing of RIZ1 prevented it. On the other hand, a p53 inhibitor enhanced the TNF-α-induced RIZ1 expression. Silencing of RIZ1 augmented the proliferative activity of TNF-α-treated cells. Therefore, it is suggested that RIZ1 negatively regulated the cell proliferation of monocytic leukemia cells via activation of p53.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Division / drug effects
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DNA Methylation
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DNA Primers
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / pharmacology*
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Gene Expression Regulation, Neoplastic
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Gene Silencing
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Histone-Lysine N-Methyltransferase / genetics
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Histone-Lysine N-Methyltransferase / pharmacology*
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Homeostasis
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Humans
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Leukemia, Myeloid / genetics
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Leukemia, Myeloid / pathology*
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Mice
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Neoplasms / genetics
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Neoplasms / pathology
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Nuclear Proteins / genetics
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Nuclear Proteins / pharmacology*
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Reverse Transcriptase Polymerase Chain Reaction
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Transcription Factors / genetics
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Transcription Factors / pharmacology*
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Transfection
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Tumor Necrosis Factor-alpha / genetics
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Tumor Suppressor Protein p53 / drug effects
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Tumor Suppressor Protein p53 / metabolism*
Substances
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DNA Primers
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DNA-Binding Proteins
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Nuclear Proteins
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Transcription Factors
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Tumor Necrosis Factor-alpha
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Tumor Suppressor Protein p53
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Histone-Lysine N-Methyltransferase
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PRDM2 protein, human