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Plant Signal Behav. 2010 Jan;5(1):1-3.

ABP1: an auxin receptor for fast responses at the plasma membrane.

Author information

1
Botanisches Institut und Botanischer Garten der Christian-Albrechts-Universität zu Kiel, Kiel, Germany. rdahlke@bot.uni-kiel.de

Abstract

Auxin-binding protein 1 (ABP1) is an auxin receptor for responses not primarily regulated by gene regulation. One fast response is protoplast swelling. By using immunological ABP1 tools we showed that the highly conserved box a is not alone important for auxin binding. Box c is another part of the auxin binding domain.1 Here we present a novel method to analyze auxin-induced, ABP1-mediated effects at the plasma membrane on single cell level in vivo. The fluorescence of FM4-64 in the plasma membrane is reduced by auxin and this response is mediated by ABP1. This method indicates a functional role of ABP1 at the plasma membrane.

KEYWORDS:

Auxin-binding protein 1; FM4-64; auxin; plasma membrane; protoplast; receptor

PMID:
20592799
PMCID:
PMC2835948
[Indexed for MEDLINE]
Free PMC Article
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