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J Clin Microbiol. 2010 Sep;48(9):3392-4. doi: 10.1128/JCM.01109-10. Epub 2010 Jun 30.

Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis.

Author information

1
Centre of Molecular Biology and Gene Therapy, Department of Internal Medicine-Hematooncology, University Hospital Brno, Cernopolni 9, 613 00 Brno, Czech Republic. khrncirova@fnbrno.cz

Abstract

We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.

PMID:
20592150
PMCID:
PMC2937726
DOI:
10.1128/JCM.01109-10
[Indexed for MEDLINE]
Free PMC Article

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