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PLoS Pathog. 2010 Jun 17;6(6):e1000954. doi: 10.1371/journal.ppat.1000954.

A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.

Author information

1
Department of Microbiology, Penn Genome Frontiers Institute, The University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America.

Abstract

Poxviruses include medically important human pathogens, yet little is known about the specific cellular factors essential for their replication. To identify genes essential for poxvirus infection, we used high-throughput RNA interference to screen the Drosophila kinome for factors required for vaccinia infection. We identified seven genes including the three subunits of AMPK as promoting vaccinia infection. AMPK not only facilitated infection in insect cells, but also in mammalian cells. Moreover, we found that AMPK is required for macropinocytosis, a major endocytic entry pathway for vaccinia. Furthermore, we show that AMPK contributes to other virus-independent actin-dependent processes including lamellipodia formation and wound healing, independent of the known AMPK activators LKB1 and CaMKK. Therefore, AMPK plays a highly conserved role in poxvirus infection and actin dynamics independent of its role as an energy regulator.

PMID:
20585561
PMCID:
PMC2887478
DOI:
10.1371/journal.ppat.1000954
[Indexed for MEDLINE]
Free PMC Article

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