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BMC Cancer. 2010 Jun 28;10:336. doi: 10.1186/1471-2407-10-336.

An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer.

Author information

1
Unidad de Investigación, Instituto de Investigación Sanitaria IdIPAZ, Hospital Universitario La Paz, Paseo de la Castellana 261, Madrid 28046, Spain.

Abstract

BACKGROUND:

Gene expression profiling may improve prognostic accuracy in patients with early breast cancer. Our objective was to demonstrate that it is possible to develop a simple molecular signature to predict distant relapse.

METHODS:

We included 153 patients with stage I-II hormonal receptor-positive breast cancer. RNA was isolated from formalin-fixed paraffin-embedded samples and qRT-PCR amplification of 83 genes was performed with gene expression assays. The genes we analyzed were those included in the 70-Gene Signature, the Recurrence Score and the Two-Gene Index. The association among gene expression, clinical variables and distant metastasis-free survival was analyzed using Cox regression models.

RESULTS:

An 8-gene prognostic score was defined. Distant metastasis-free survival at 5 years was 97% for patients defined as low-risk by the prognostic score versus 60% for patients defined as high-risk. The 8-gene score remained a significant factor in multivariate analysis and its performance was similar to that of two validated gene profiles: the 70-Gene Signature and the Recurrence Score. The validity of the signature was verified in independent cohorts obtained from the GEO database.

CONCLUSIONS:

This study identifies a simple gene expression score that complements histopathological prognostic factors in breast cancer, and can be determined in paraffin-embedded samples.

PMID:
20584321
PMCID:
PMC2906483
DOI:
10.1186/1471-2407-10-336
[Indexed for MEDLINE]
Free PMC Article

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