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Biomol NMR Assign. 2010 Oct;4(2):207-9. doi: 10.1007/s12104-010-9244-7. Epub 2010 Jun 29.

Backbone assignment of the catalytic core of a Y-family DNA polymerase.

Author information

1
Department of Biochemistry, The Ohio State University, 880 Biological Sciences, 484 West 12th Ave., Columbus, OH 43210, USA.

Abstract

Sulfolobus solfataricus DNA polymerase IV (Dpo4), a model Y-family DNA polymerase, bypasses DNA lesions. Here, we report the assignments for the backbone nitrogen, carbon, and amide proton NMR signals of Dpo4's catalytic core consisting of the finger, palm, and thumb domains. Our work provides the basis for further NMR spectroscopic studies of the interactions among Dpo4, DNA, and an incoming nucleotide.

PMID:
20582735
DOI:
10.1007/s12104-010-9244-7
[Indexed for MEDLINE]

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