Abstract
Escherichia coli transforms the methanogenic archaeon Methanococcus maripaludis at frequencies ranging from 0.2 x 10(-6) to 2 x 10(-6) per recipient cell. Transformation requires cell-to-cell contact, oriT, and tra functions, is insensitive to DNase I, and otherwise displays hallmarks of conjugation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Conjugation, Genetic*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics*
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Escherichia coli / genetics*
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Gene Transfer Techniques
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Gene Transfer, Horizontal*
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Genes, Bacterial
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Genetic Vectors
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Methanococcus / genetics*
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Molecular Sequence Data
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Plasmids
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Sequence Analysis, DNA