Interdomain conjugal transfer of DNA from bacteria to archaea

Appl Environ Microbiol. 2010 Aug;76(16):5644-7. doi: 10.1128/AEM.00967-10. Epub 2010 Jun 25.

Abstract

Escherichia coli transforms the methanogenic archaeon Methanococcus maripaludis at frequencies ranging from 0.2 x 10(-6) to 2 x 10(-6) per recipient cell. Transformation requires cell-to-cell contact, oriT, and tra functions, is insensitive to DNase I, and otherwise displays hallmarks of conjugation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Conjugation, Genetic*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics*
  • Escherichia coli / genetics*
  • Gene Transfer Techniques
  • Gene Transfer, Horizontal*
  • Genes, Bacterial
  • Genetic Vectors
  • Methanococcus / genetics*
  • Molecular Sequence Data
  • Plasmids
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial

Associated data

  • GENBANK/HM536627