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J Biol Chem. 2010 Sep 3;285(36):28220-8. doi: 10.1074/jbc.M110.118554. Epub 2010 Jun 25.

Clostridiolysin S, a post-translationally modified biotoxin from Clostridium botulinum.

Author information

1
Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California 92093, USA.

Abstract

Through elaboration of its botulinum toxins, Clostridium botulinum produces clinical syndromes of infant botulism, wound botulism, and other invasive infections. Using comparative genomic analysis, an orphan nine-gene cluster was identified in C. botulinum and the related foodborne pathogen Clostridium sporogenes that resembled the biosynthetic machinery for streptolysin S, a key virulence factor from group A Streptococcus responsible for its hallmark beta-hemolytic phenotype. Genetic complementation, in vitro reconstitution, mass spectral analysis, and plasmid intergrational mutagenesis demonstrate that the streptolysin S-like gene cluster from Clostridium sp. is responsible for the biogenesis of a novel post-translationally modified hemolytic toxin, clostridiolysin S.

PMID:
20581111
PMCID:
PMC2934687
DOI:
10.1074/jbc.M110.118554
[Indexed for MEDLINE]
Free PMC Article

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