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J Biol Chem. 2010 Aug 27;285(35):26916-22. doi: 10.1074/jbc.M110.123083. Epub 2010 Jun 24.

Defining specific lipid binding sites for a peripheral membrane protein in situ using subtesla field-cycling NMR.

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1
Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02465, USA.

Abstract

Despite the profound physiological consequences associated with peripheral membrane protein localization, only a rudimentary understanding of the interactions of proteins with membrane surfaces exists because these questions are inaccessible by commonly used structural techniques. Here, we combine high resolution field-cycling (31)P NMR relaxation methods with spin-labeled proteins to delineate specific interactions of a bacterial phospholipase C with phospholipid vesicles. Unexpectedly, discrete binding sites for both a substrate analogue and a different phospholipid (phosphatidylcholine) known to activate the enzyme are observed. The lifetimes for the occupation of these sites (when the protein is anchored transiently to the membrane) are >1-2 micros (but <1 ms), which represents the first estimate of an off-rate for a lipid dissociating from a specific site on the protein and returning to the bilayer. Furthermore, analyses of the spin-label induced NMR relaxation corroborates the presence of a discrete tyrosine-rich phosphatidylcholine binding site whose location is consistent with that suggested by modeling studies. The methodology illustrated here may be extended to a wide range of peripheral membrane proteins.

PMID:
20576615
PMCID:
PMC2930691
DOI:
10.1074/jbc.M110.123083
[Indexed for MEDLINE]
Free PMC Article
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