Dengue-2-virus-interacting polypeptides involved in mosquito cell infection

Arch Virol. 2010 Sep;155(9):1453-61. doi: 10.1007/s00705-010-0728-7. Epub 2010 Jun 23.

Abstract

For the design of effective antiviral strategies, understanding the fundamental steps of the virus life cycle, including virus-host interactions, is essential. We performed a virus overlay protein binding assay followed by proteomics for identification of proteins from membrane fractions of A7 (Aedes aegypti) cells, C6/36 (Aedes albopictus) cells and the midgut brush border membrane fraction of Ae. aegypti mosquito that bind to dengue-2 virus. Actin, ATP synthase β subunit, HSc 70, orisis, prohibitin, tubulin β chain, and vav-1 were identified as dengue-2-virus-binding proteins. Our results suggest that dengue-2 virus exploits an array of housekeeping proteins for its entry in mosquito cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes / genetics
  • Aedes / metabolism*
  • Aedes / virology
  • Animals
  • Cell Line
  • Dengue
  • Dengue Virus / genetics
  • Dengue Virus / physiology*
  • Insect Proteins / genetics
  • Insect Proteins / metabolism*
  • Insect Vectors / genetics
  • Insect Vectors / metabolism*
  • Insect Vectors / virology
  • Molecular Sequence Data
  • Peptides / genetics
  • Peptides / metabolism*
  • Virus Internalization

Substances

  • Insect Proteins
  • Peptides