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Chem Commun (Camb). 2010 Aug 14;46(30):5506-8. doi: 10.1039/c0cc00108b. Epub 2010 Jun 22.

A method to site-specifically introduce methyllysine into proteins in E. coli.

Author information

1
Department of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.

Abstract

A mutant pyrrolysyl-tRNA synthetase/tRNA pair was used to genetically encode allylcarbamoyl methyllysine in bacteria. This amino acid can be converted to methyllysine with a ruthenium catalyst, providing a straightforward approach for site-specifically introducing methyllysine residues into proteins.

PMID:
20571694
PMCID:
PMC2928331
DOI:
10.1039/c0cc00108b
[Indexed for MEDLINE]
Free PMC Article

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