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Malar J. 2010 Jun 17;9:170. doi: 10.1186/1475-2875-9-170.

New synchronization method for Plasmodium falciparum.

Author information

1
Division of Infection and Immunity, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre, Glasgow G12 8TA, Scotland. L.ranford-cartwright@bio.gla.ac.uk

Abstract

BACKGROUND:

Plasmodium falciparum is usually asynchronous during in vitro culture. Although various synchronization methods are available, they are not able to narrow the range of ages of parasites. A newly developed method is described that allows synchronization of parasites to produce cultures with an age range as low as 30 minutes.

METHODS:

Trophozoites and schizonts are enriched using Plasmion. The enriched late stage parasites are immobilized as a monolayer onto plastic Petri dishes using concanavalin A. Uninfected erythrocytes are placed onto the monolayer for a limited time period, during which time schizonts on the monolayer rupture and the released merozoites invade the fresh erythrocytes. The overlay is then taken off into a culture flask, resulting in a highly synchronized population of parasites.

RESULTS:

Plasmion treatment results in a 10- to 13-fold enrichment of late stage parasites. The monolayer method results in highly synchronized cultures of parasites where invasion has occurred within a very limited time window, which can be as low as 30 minutes. The method is simple, requiring no specialized equipment and relatively cheap reagents.

CONCLUSIONS:

The new method for parasite synchronization results in highly synchronized populations of parasites, which will be useful for studies of the parasite asexual cell cycle.

PMID:
20565741
PMCID:
PMC2901355
DOI:
10.1186/1475-2875-9-170
[Indexed for MEDLINE]
Free PMC Article

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