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Nat Neurosci. 2010 Jul;13(7):869-76. doi: 10.1038/nn.2578. Epub 2010 Jun 20.

Hearing requires otoferlin-dependent efficient replenishment of synaptic vesicles in hair cells.

Author information

1
InnerEarLab, Department of Otolaryngology and Center for Molecular Physiology of the Brain, University Medical Center Göttingen, Göttingen, Germany.

Abstract

Inner hair cell ribbon synapses indefatigably transmit acoustic information. The proteins mediating their fast vesicle replenishment (hundreds of vesicles per s) are unknown. We found that an aspartate to glycine substitution in the C(2)F domain of the synaptic vesicle protein otoferlin impaired hearing by reducing vesicle replenishment in the pachanga mouse model of human deafness DFNB9. In vitro estimates of vesicle docking, the readily releasable vesicle pool (RRP), Ca(2+) signaling and vesicle fusion were normal. Moreover, we observed postsynaptic excitatory currents of variable size and spike generation. However, mutant active zones replenished vesicles at lower rates than wild-type ones and sound-evoked spiking in auditory neurons was sparse and only partially improved during longer interstimulus intervals. We conclude that replenishment does not match the release of vesicles at mutant active zones in vivo and a sufficient standing RRP therefore cannot be maintained. We propose that otoferlin is involved in replenishing synaptic vesicles.

PMID:
20562868
DOI:
10.1038/nn.2578
[Indexed for MEDLINE]

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