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EMBO J. 2010 Jul 21;29(14):2315-28. doi: 10.1038/emboj.2010.133. Epub 2010 Jun 18.

Cooperative interactions at the SLP-76 complex are critical for actin polymerization.

Author information

1
Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel. bardasm@mail.biu.ac.il

Abstract

T-cell antigen receptor (TCR) engagement induces formation of multi-protein signalling complexes essential for regulating T-cell functions. Generation of a complex of SLP-76, Nck and VAV1 is crucial for regulation of the actin machinery. We define the composition, stoichiometry and specificity of interactions in the SLP-76, Nck and VAV1 complex. Our data reveal that this complex can contain one SLP-76 molecule, two Nck and two VAV1 molecules. A direct interaction between Nck and VAV1 is mediated by binding between the C-terminal SH3 domain of Nck and the VAV1 N-terminal SH3 domain. Disruption of the VAV1:Nck interaction deleteriously affected actin polymerization. These novel findings shed new light on the mechanism of actin polymerization after T-cell activation.

PMID:
20562827
PMCID:
PMC2910278
DOI:
10.1038/emboj.2010.133
[Indexed for MEDLINE]
Free PMC Article

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