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Org Biomol Chem. 2010 Aug 7;8(15):3398-401. doi: 10.1039/c000158a. Epub 2010 Jun 16.

Fura-2FF-based calcium indicator for protein labeling.

Author information

1
Laboratory of Protein Engineering, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

Abstract

We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca(2+) sensing ability after coupling to protein.

PMID:
20556282
DOI:
10.1039/c000158a
[Indexed for MEDLINE]

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