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Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2010 Sep;27(9):1261-5. doi: 10.1080/19440049.2010.487501.

Determination of aflatoxin M1 in milk by triple quadrupole liquid chromatography-tandem mass spectrometry.

Author information

1
China National Food Quality and Safety Supervision and Inspection Center, Haidian District Institute of Products Quality Supervision and Inspection, Beijing, China.

Abstract

A sensitive and reliable method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with an electrospray-positive ionization method was developed for the determination of aflatoxin M(1) in milk. This method includes simple extraction of the sample with acetonitrile by ultrasonic, separation on an MGIII-C(18) column using 0.01% formic acid buffer/acetonitrile (60 : 40, v/v) as mobile phase, and MS/MS detection using multiple reaction-monitoring mode. Average recoveries of aflatoxin M(1) from spiked samples at concentrations of 0.02 and 1 ng ml(-1) ranged from 77% to 94%, with a 6% relative standard deviation. The limit of detection and limit of quantification were 0.006 and 0.02 ng ml(-1), respectively. The standard curve was linear between 0.02 and 20.0 ng ml(-1). The recommended method is simple, rapid, specific and reliable for the routine monitoring of aflatoxin M(1) in milk.

PMID:
20552474
DOI:
10.1080/19440049.2010.487501
[Indexed for MEDLINE]

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