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J Math Biol. 2011 May;62(5):685-706. doi: 10.1007/s00285-010-0350-z. Epub 2010 Jun 12.

Derivation, identification and validation of a computational model of a novel synthetic regulatory network in yeast.

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1
Telethon Institute of Genetics and Medicine (TIGEM), 80131 Naples, Italy. marucci@tigem.it

Abstract

Systems biology aims at building computational models of biological pathways in order to study in silico their behaviour and to verify biological hypotheses. Modelling can become a new powerful method in molecular biology, if correctly used. Here we present step-by-step the derivation and identification of the dynamical model of a biological pathway using a novel synthetic network recently constructed in the yeast Saccharomyces cerevisiae for In-vivo Reverse-Engineering and Modelling Assessment. This network consists of five genes regulating each other transcription. Moreover, it includes one protein-protein interaction, and its genes can be switched on by addition of galactose to the medium. In order to describe the network dynamics, we adopted a deterministic modelling approach based on non-linear differential equations. We show how, through iteration between experiments and modelling, it is possible to derive a semi-quantitative prediction of network behaviour and to better understand the biology of the pathway of interest.

PMID:
20549211
DOI:
10.1007/s00285-010-0350-z
[Indexed for MEDLINE]
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