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J Physiol. 2010 Aug 1;588(Pt 15):2945-60. doi: 10.1113/jphysiol.2010.191593. Epub 2010 Jun 14.

In vivo expression of G-protein beta1gamma2 dimer in adult mouse skeletal muscle alters L-type calcium current and excitation-contraction coupling.

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1
Université Lyon 1, UMR CNRS 5123, Physiologie Intégrative Cellulaire et Moléculaire, Bâtiment R. Dubois, 43 boulevard du 11 novembre 1918, Villeurbanne, France. norbert.weiss@yahoo.fr

Abstract

A number of G-protein-coupled receptors are expressed in skeletal muscle but their roles in muscle physiology and downstream effector systems remain poorly investigated. Here we explored the functional importance of the G-protein betagamma (Gbetagamma) signalling pathway on voltage-controlled Ca(2+) homeostasis in single isolated adult skeletal muscle fibres. A GFP-tagged Gbeta(1)gamma(2) dimer was expressed in vivo in mice muscle fibres. The GFP fluorescence pattern was consistent with a Gbeta(1)gamma(2) dimer localization in the transverse-tubule membrane. Membrane current and indo-1 fluorescence measurements performed under voltage-clamp conditions reveal a drastic reduction of both L-type Ca(2+) current density and of peak amplitude of the voltage-activated Ca(2+) transient in Gbeta(1)gamma(2)-expressing fibres. These effects were not observed upon expression of Gbeta(2)gamma(2), Gbeta(3)gamma(2) or Gbeta(4)gamma(2). Our data suggest that the G-protein beta(1)gamma(2) dimer may play an important regulatory role in skeletal muscle excitation-contraction coupling.

PMID:
20547679
PMCID:
PMC2956909
DOI:
10.1113/jphysiol.2010.191593
[Indexed for MEDLINE]
Free PMC Article
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