Monitoring biofilm development in a microfluidic device using modified confocal reflection microscopy

Yutaka Yawata; Kensuke Toda; Erika Setoyama; Junji Fukuda; Hiroaki Suzuki; Hiroo Uchiyama; Nobuhiko Nomura

doi:10.1016/j.jbiosc.2010.04.002

Monitoring biofilm development in a microfluidic device using modified confocal reflection microscopy

J Biosci Bioeng. 2010 Sep;110(3):377-80. doi: 10.1016/j.jbiosc.2010.04.002. Epub 2010 May 5.

Authors

Yutaka Yawata¹, Kensuke Toda, Erika Setoyama, Junji Fukuda, Hiroaki Suzuki, Hiroo Uchiyama, Nobuhiko Nomura

Affiliation

¹ Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennnoudai1-1-1, Tsukuba, Ibaraki 305-8572, Japan.

PMID: 20547370
DOI: 10.1016/j.jbiosc.2010.04.002

Abstract

The feasibility of a method to monitor biofilm development non-destructively in a microfluidic device was addressed. Here, we report that biofilm growth could be non-destructively monitored by an image analysis technique based on modification of confocal reflection microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Load / instrumentation
Bacterial Load / methods*
Biofilms / growth & development*
Bioreactors
Cell Culture Techniques
Image Interpretation, Computer-Assisted / methods*
Microfluidic Analytical Techniques / instrumentation
Microfluidic Analytical Techniques / methods*
Microscopy, Confocal / methods*
Streptococcus mutans / cytology*
Streptococcus mutans / growth & development*