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J Biotechnol. 2010 Aug 2;148(4):204-7. doi: 10.1016/j.jbiotec.2010.06.008. Epub 2010 Jun 11.

Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes.

Author information

1
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan. honda@bio.eng.osaka-u.ac.jp

Abstract

Six thermophilic enzymes from Thermus thermophilus were used to construct an 'artificial bio-synthetic pathway' for the production of 2-deoxyribose 5-phosphate from fructose. By a simple operation using six recombinant Escherichia coli strains producing the thermophilic enzymes, respectively, fructose was converted to 2-deoxyribose 5-phosphate with a molar yield of 55%.

PMID:
20547190
DOI:
10.1016/j.jbiotec.2010.06.008
[Indexed for MEDLINE]

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