A Biomimetic Material with a High Bio-responsibility for Bone Reconstruction and Tissue Engineering

J Biomater Sci Polym Ed. 2011;22(1-3):153-63. doi: 10.1163/092050609X12583524936191.

Abstract

A biomimetic composite was prepared using type-I collagen as the matrix, and particles of sol-gel-derived bioactive glass (58S), hyaluronic acid and phosphatidylserine as additives. The material has an interconnected 3-D porous structure with a porosity > 85%. When incubated in simulated body fluid (SBF), the composite induced the formation of microcrystals of bone-like hydroxyapatite (HA), suggesting good bioactive properties. During the in vitro cell-culture experiment, MC3T3-E1 cells adhered to, migrated and spread on the surface of the porous composite. The material was employed to repair a 10-mm defect in a rabbit's radius. The composite was gradually degraded within 8 weeks and replaced by new bone. After 12 weeks, the bone marrow cavity was restored and the Haversian canal was noted from the histological observation. The biomimetic composite is a potential scaffold material for bone reconstruction and bone tissue engineering.

Keywords: BIO-MINERALIZATION; BIOACTIVE GLASS; BIOMIMETIC; BONE RECONSTRUCTION; COLLAGEN.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Absorbable Implants*
  • Animals
  • Biomimetic Materials* / chemistry
  • Bone Regeneration
  • Bone Substitutes* / chemistry
  • Calcification, Physiologic
  • Cell Adhesion
  • Cell Movement
  • Disease Models, Animal
  • Durapatite / chemistry
  • Glass* / chemistry
  • Mice
  • Microscopy, Electron, Scanning
  • Porosity
  • Rabbits
  • Radius / injuries
  • Spectrum Analysis
  • Tissue Engineering / instrumentation
  • Tissue Scaffolds* / chemistry
  • X-Ray Diffraction

Substances

  • Bone Substitutes
  • Durapatite