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Biochim Biophys Acta. 1991 Jun 13;1089(2):241-3.

Specific hybridization probes for mouse type I, II, III and IX collagen mRNAs.

Author information

1
University of Texas M.D. Anderson Cancer Center, Department of Molecular Genetics, Houston.

Abstract

We have constructed DNA probes for the specific detection of mouse pro alpha 1(I), pro alpha 1(II), pro alpha 1(III) and alpha 1(IX) collagen transcripts. To avoid cross-hybridization the probes for fibrillar collagens cover mainly sequences in the 3' untranslated region of the gene. Sequencing and Northern analysis confirmed that the clones share minimal sequence similarity and detect only the specific mRNAs under normal hybridization and washing conditions. The clone for mouse alpha 1(IX) collagen covers coding sequences but is sufficiently divergent from other collagen transcripts to allow specific detection of the corresponding mRNA.

PMID:
2054384
DOI:
10.1016/0167-4781(91)90014-d
[Indexed for MEDLINE]

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