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J Antimicrob Chemother. 2010 Aug;65(8):1583-5. doi: 10.1093/jac/dkq199. Epub 2010 Jun 11.

Depletion of resuscitation-promoting factors has limited impact on the drug susceptibility of Mycobacterium tuberculosis.

Author information

1
MRC/NHLS/WITS Molecular Mycobacteriology Research Unit, DST/NRF Centre of Excellence for Biomedical TB Research, University of the Witwatersrand, National Health Laboratory Service, Johannesburg 2000, South Africa. bavesh.kana@nhls.ac.za

Abstract

OBJECTIVES:

Mycobacterium tuberculosis has five homologues of the muralytic resuscitation-promoting factor (Rpf), which are collectively dispensable for growth in vitro, but are required for resuscitation from a non-culturable state and for virulence in a mouse infection model. Our study was aimed at assessing the drug susceptibility of mutants lacking all five rpf genes.

METHODS:

Drug susceptibility was determined in liquid medium using the broth microdilution method.

RESULTS:

Strains of M. tuberculosis that lack all five rpf-like genes display a 4-8-fold increase in susceptibility to vancomycin and erythromycin, but show no significant difference in susceptibility to other antibiotics tested, including first- and second-line antitubercular agents.

CONCLUSIONS:

These results suggest that the possible alterations in the peptidoglycan structure and/or turnover caused by Rpf deficiency have a limited impact on the drug susceptibility of M. tuberculosis.

PMID:
20542905
DOI:
10.1093/jac/dkq199
[Indexed for MEDLINE]

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