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J Antimicrob Chemother. 2010 Aug;65(8):1586-8. doi: 10.1093/jac/dkq180. Epub 2010 Jun 11.

Novel genetic context of multiple bla OXA-58 genes in Acinetobacter genospecies 3.

Author information

1
Faculty of Life Sciences, The University of Manchester, Manchester, UK.

Abstract

OBJECTIVES:

The detection in Acinetobacter genospecies 3 isolates of OXA-type carbapenemases, resulting in reduced susceptibility to carbapenem antibiotics, is increasingly reported. We identified an Acinetobacter genospecies 3 isolate carrying the gene for OXA-58 and aimed to resolve the genetic environment surrounding the bla(OXA-58) gene.

METHODS:

Species identification was confirmed by 16S-23S rRNA restriction analysis. MICs of imipenem, meropenem and ertapenem were determined, and the isolate was screened by PCR for bla(OXA-23-like), bla(OXA-40-like), bla(OXA-51-like) and bla(OXA-58-like) genes. The sequence surrounding bla(OXA-58) was determined through amplification by inverse PCR and genome walking followed by sequencing. Genetic localization was investigated by Southern blotting.

RESULTS:

Isolate A164 was confirmed as belonging to Acinetobacter genospecies 3 and had reduced susceptibility to the carbapenems. The isolate was found to encode two bla(OXA-58) genes that may have been duplicated by the insertion sequence ISAba125, two copies of which were inserted into ISAba3 elements. The bla(OXA-58) genes appear to be plasmid borne.

CONCLUSIONS:

This is the first report of beta-lactamase duplication in Acinetobacter genospecies 3 and of gene duplication mediated by ISAba125.

PMID:
20542900
DOI:
10.1093/jac/dkq180
[Indexed for MEDLINE]

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