Format

Send to

Choose Destination
Reprod Biomed Online. 2010 Aug;21(2):196-205. doi: 10.1016/j.rbmo.2010.04.017. Epub 2010 Apr 24.

Erythropoietic differentiation of a human embryonic stem cell line harbouring the sickle cell anaemia mutation.

Author information

1
Institute for Cell Engineering, Stem Cell Program, Johns Hopkins University School of Medicine, 733 N Broadway, BRB 755 Baltimore, MD 21205, USA.

Abstract

Herein is reported efficient erythropoietic differentiation of a human embryonic stem cell (ESC) line derived from a preimplantation genetic diagnosis (PGD)-screened embryo that harbours the homozygous sickle cell disease (SCD) haemoglobinopathy mutation. This human ESC line possesses typical pluripotency characteristics and forms multilineage teratomas in vivo. SCD-human ESC efficiently differentiated to the haematopoietic lineage under serum-free and stromal co-culture conditions and gave rise to robust primitive and definitive erythrocytes. Expression of embryonic, fetal and adult sickle globin genes in SCD PGD-derived human ESC-derived erythrocytes was confirmed by quantitative real-time PCR, intracytoplasmic fluorescence-activated cell sorting and in-situ immunostaining of PGD-derived human ESC teratoma sections. These data introduce important methodologies and paradigms for using patient-specific human ESC to generate normal and haemoglobinopathic erythroid progenitors for biomedical research.

PMID:
20541472
PMCID:
PMC3507446
DOI:
10.1016/j.rbmo.2010.04.017
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center