Rapid and accurate method for isolation of recombinant baculovirus with an expanded host range

J Biosci Bioeng. 2010 Jul;110(1):66-8. doi: 10.1016/j.jbiosc.2009.12.007. Epub 2010 Jan 18.

Abstract

A rapid positive screening system for identifying recombinant baculovirus with an expanded host range was developed by using an actin promoter-dependent fluorescent protein gene expression cassette. The expression of recombinant protein regulated by the polyhedrin promoter was not affected by the insertion of the cassette in the baculovirus genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Baculoviridae / genetics*
  • Baculoviridae / isolation & purification*
  • Gene Expression Regulation, Viral
  • Mutagenesis, Insertional
  • Recombinant Proteins / genetics
  • Virology / methods*

Substances

  • Recombinant Proteins