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J Biotechnol. 2010 Aug 2;148(4):182-93. doi: 10.1016/j.jbiotec.2010.06.005. Epub 2010 Jun 9.

Global assessment of protein turnover in recombinant antibody producing myeloma cells.

Author information

1
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455-0132, USA.

Abstract

The global turnover rates of cellular proteins and the secretion rate of a recombinant immunoglobulin G (IgG) in a myeloma cell line, NS0, were determined using SILAC proteomic analysis. After complete labeling of cellular proteins with (13)C(6), (15)N(4)-arginine, cells were transferred to unlabeled medium and the decay of the labeled arginine in proteins was monitored during exponential cell growth. After PAGE separation and mass-spectrometric identification of proteins, those detected with high confidence over at least three time points were used for the determination of turnover rates. Among the 224 proteins quantified with a protein half-life, about 15% have a degradation rate constant lower than one-tenth of specific growth rate. For most proteins, the turnover rate is insignificant in its overall dynamics. Only 6.3% of proteins have a half-life shorter than the cell doubling time. For IgG secretion, both heavy and light chain molecules follow the same kinetic behavior with a half-life estimated to be 2h. The label decay curve appears to show a second region with very slow kinetics, raising the possibility of two populations of IgG molecules with different secretion characteristics.

PMID:
20540971
DOI:
10.1016/j.jbiotec.2010.06.005
[Indexed for MEDLINE]

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