Format

Send to

Choose Destination
See comment in PubMed Commons below
Virol J. 2010 Jun 10;7:123. doi: 10.1186/1743-422X-7-123.

Viral trans-factor independent replication of human papillomavirus genomes.

Author information

1
Nebraska Center for Virology, School of Biological Sciences, University of Nebraska Lincoln, Lincoln, NE 68583-0900, USA.

Abstract

BACKGROUND:

Papillomaviruses (PVs) establish a persistent infection in the proliferating basal cells of the epithelium. The viral genome is replicated and maintained as a low-copy nuclear plasmid in basal keratinocytes. Bovine and human papillomaviruses (BPV and HPV) are known to utilize two viral proteins; E1, a DNA helicase, and E2, a transcription factor, which have been considered essential for viral DNA replication. However, growing evidence suggests that E1 and E2 are not entirely essential for stable replication of HPV.

RESULTS:

Here we report that multiple HPV16 mutants, lacking either or both E1 and E2 open reading frame (ORFs) and the long control region (LCR), still support extrachromosomal replication. Our data clearly indicate that HPV16 has a mode of replication, independent of viral trans-factors, E1 and E2, which is achieved by origin activity located outside of the LCR.

PMID:
20537170
PMCID:
PMC2893153
DOI:
10.1186/1743-422X-7-123
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for BioMed Central Icon for PubMed Central
    Loading ...
    Support Center