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Biosci Biotechnol Biochem. 2010;74(6):1315-9. Epub 2010 Jun 7.

Gateway binary vectors with the bialaphos resistance gene, bar, as a selection marker for plant transformation.

Author information

1
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Matsue, Japan.

Abstract

We constructed two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing the bialaphos resistance gene (bar) as a selection marker for plant transformation. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7 and TAP. Selection of Arabidopsis transformants with BASTA was successfully carried out using both plate-grown and soil-grown seedlings. Transformed rice calli and suspension-cultured tobacco cells were selected on plates containing BASTA or glufosinate-ammonium. These vectors are compatible with existing pGWB and R4pGWB vectors carrying kanamycin and hygromycin B resistance.

PMID:
20530878
DOI:
10.1271/bbb.100184
[Indexed for MEDLINE]
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