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Nat Methods. 2010 Jul;7(7):508-11. doi: 10.1038/nmeth.1467. Epub 2010 Jun 6.

Proteome-wide analysis of protein carboxy termini: C terminomics.

Author information

1
Department of Biochemistry and Molecular Biology, University of British Columbia, Centre for Blood Research, Vancouver, British Columbia, Canada.

Abstract

As proteome-wide C-terminal sequence analysis has been largely intractable, we developed a polymer-based enrichment approach to profile protein C-terminal peptides by mass spectrometry and identified hundreds of C-terminal peptides in the Escherichia coli proteome. We isotopically labeled GluC protease-digested and undigested samples and identified GluC substrates and their cleavage sites by quantification of neo-C-terminal peptides. Our method thus enables global annotation of protein C-terminal posttranslational modifications, including proteolytic truncations.

PMID:
20526347
DOI:
10.1038/nmeth.1467
[Indexed for MEDLINE]

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